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pmol/ g FW during nodule formation. Levels of 12-oxophytodienoic acid expressed per mg
of protein presented a five-fold increase during plantlet regeneration (a higher increase than
that detected for jasmonic acid) suggesting that it may play a different role from jasmonic
acid in the process. Double immunolocalization experiments with an antibody raised
against Rubisco together with Lugol staining showed that allene oxide cyclase (AOC) is
present in amyloplasts of prenodular cells and in chloroplasts of vacuolated nodular cells
(Fig. 3) whereas meristematic cells showed little allene oxide cyclase accumulation. The
presence of allene oxide cyclase in non-photosynthetic tissues may be related to the ability
of jasmonic acid to stimulate carbon and nitrogen accumulation that will be used later in
developmental processes.
References
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Fortes AM, Pais MS (2000). Am. J. Bot. 87 (7), 971-979.
[2]
Fortes AM, Testillano P, Risueño MC, Pais MS (2002). Physiol. Plant. 116, 113-120.
[3]
Fortes AM, Coronado MJ, Testillano P, Risueño MC, Pais MS (2003). J. Histochem. Citochem. 52 (2),
227-241.
[4]
Altschul SF, Madden TL, et al. (1997). Nucleic Acids Res. 25, 3389-3402.
[5]
Fortes AM, Miersch O, et al. (2003). DGF symposium on Plant Oxylipins, Goettingen, Germany, pp.
40.
