Chemistry Reference
In-Depth Information
Table 2.4 growth of bifidobacteria on scFos and Inulin
strain
(n)
scFos growth
(A600)
Inulin growth
(A600)
species
Difference
B. boum
1
0.256
0.024
0.232
B. pseudolongum
1
0.542
-0.051
0.593
B. globosum
1
0.659
-0.092
0.751
B. pullorum
1
0.774
-0.068
0.842
B. ruminantium
1
0.889
-0.106
1.049
B. choerinum
1
0.900
-0.214
1.114
B. animalis
2
0.957
-0.016
0.973
B. gallinarum
1
1.09
0.071
1.019
B. bifidum
8
1.10
-0.032
1.132
B. breve
2
1.13
-0.070
1.200
B. longum
6
1.18
0.002
1.178
B. species
4
1.21
0.045
1.165
B. suis
1
1.22
-0.051
1.271
B. breve/longum
1
1.28
0.029
1.251
B. merycicum
1
1.43
0.042
1.388
B. magnum
1
1.47
-0.051
1.521
B. adolescentis
2
1.54
0.041
1.499
B. infantis
2
1.57
0.300
1.270
B. minimum
1
1.85
0.670
1.180
B. cuniculi
1
2.05
0.578
1.472
B. thermophilum
4
2.13
0.390
1.740
43
average growth
average growth
1.258
a
0.0937
1.164
a
significantly different,
p
≤ 0.05.
Source:
adapted from Mckellar et al., 1993.
(Kaplan and Hutkins 2000). Similarly McKellar and Modler (1989) explored the
relationship between chain length and β-fructosidase activity in various bifidobac-
teria species (
(Bifidobacterium adolescentis
ATCC 15703,
B. longum
ATCC 15070,
B. thermophilum
ATCC 25525) and observed maximum cell-associated enzyme
activity for scFOS versus inulin. This suggests that at least some bifidobacteria and
lactobacilli selectively use different fructans according to chain length, and that
scFOS, particularly the smaller scFOS chains, are selectively utilized by certain
bacteria. FOS transporters have been identified on
L. paracasei
1995 (Kaplan and
Hutkins, 2003) and
L. acidophilus
(Barrangou et al., 2003). Transporter assays sug-
gest that FOS transport is selective for chain length, as studies with
L. paracasei
1195 revealed that the uptake of GF
2
and GF
3
was rapid, whereas little GF
4
uptake
occurred (Kaplan and Hutkins, 2003). Selective transport could explain selective
utilization of the shorter chains, specifically GF
2
and GF
3
.
