Chemistry Reference
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percentage in the total number of fecal bacteria increased significantly compared
with the levels before intake.
Tuohy et al.
3
conducted a double-blind, placebo-controlled study with 20
healthy European volunteers to investigate the effect of consumption of two 65 mL
bottles of fermented milk (6.5 × 10
9
CFU of
L . casei
Shirota/bottle) for 3 weeks on
the survival of the probiotic in the gastrointestinal tract. After 7 days of fermented
drink intake,
L. casei
Shirota was recovered from the test group's fecal samples at
10
7.1 ± 0.4
CFU/g feces (mean ± SD) and numbers were maintained at this level for 3
weeks (Figure 9.2).
Spanhaak et al.
4
performed a similar study to assess the effect of consumption
of a fermented drink containing
L . casei
Shirota in healthy Europeans. The treat-
ment group (
n
= 10) received 100 mL of a fermented milk containing 1.0 × 10
9
CFU/mL three times a day, while the control group (
n
= 10) was given the same
amount of unfermented milk (placebo) in the same manner. As a result, more
than 1.0 × 10
7
CFU/g feces of
L . casei
Shirota were recovered, and the significant
increase of
Bifidobacterium
was found in comparison to the placebo group.
Shioiri et al.
5
investigated the effect of consumption of
L. casei
Shirota and
transgalactosylated oligosaccharides on the microflora of elderly Japanese sub-
jects. The volunteers were administered a fermented milk beverage containing
L. casei
Shirota at 3.0 × 10
10
CFU/bottle and 2.5 g of transgalactosylated oli-
gosaccharides once a day for 2 weeks. By weeks 1 and 2 of ingestion of the fer-
mented milk beverage, the numbers of fecal
Bifidobacterium
and
Lactobacillus
were significantly higher than those of the placebo group. On the other hand, the
numbers of fecal lecithinase-positive
Clostridium
and Enterobacteriaceae in the
8
6
4
2
Intake of fermented milk
0
7 14 21 28
(Days)
Figure 9.2
survivability of
L. casei
shirota in feces by drinking of a fermented milk bever-
age containing
L. casei
shirota.
Healthy subjects drank two 65-mL bottles of
fermented milk for 3 weeks. on days 7, 14, 21, and 28 after subjects stopped
drinking sample for 7 days, the fecal numbers of
L. casei
shirota were measured.
each black circle represents the mean value of Log
10
Cfu/g feces, and each
bar expresses standard deviations (error bar). (from tuohy, k.M. et al.,
J. Appl.
Microbiol
., 102, 1026-1032, 2007. With permission.
3
)
