Environmental Engineering Reference
In-Depth Information
7.3.2
Soil characterization...................................................................... 243
7.3.3
Flask (bench-scale) experimental design................................... 244
7.3.4
Flask (bench-scale) materials....................................................... 244
7.3.4.1
Bacteria ............................................................................. 244
7.3.4.2
Amendments ................................................................... 244
7.3.5
Flask (bench-scale) methods........................................................ 245
7.3.5.1
Isolation and characterization of
PAH-degrading bacteria ................................................ 245
7.3.5.2
Biosurfactant production............................................... 247
7.3.5.3
Bioaugmentation............................................................. 248
7.3.5.4
Carrier technology development ................................. 249
7.3.5.5
Biostimulation ................................................................. 251
7.3.5.6
Microcosm preparation.................................................. 252
7.3.5.7
Microbial analysis........................................................... 252
7.3.5.8
Chemical analysis ........................................................... 252
7.3.6
Pilot studies: experimental design ............................................. 253
7.3.6.1
LTU objectives ................................................................. 255
7.3.6.2
Trough study objectives................................................. 255
7.3.7
Land treatment units: assembly ................................................. 255
7.3.8
Trough study: assembly ............................................................... 255
7.3.9
Pilot-scale materials ...................................................................... 257
7.3.10
Pilot-scale methods ....................................................................... 259
7.3.10.1 Sampling design ............................................................. 259
7.3.10.2 Physical analysis ............................................................. 260
7.3.10.3 Chemical analysis ........................................................... 260
7.3.10.4 Microbial analysis........................................................... 261
7.3.10.5 Metabolic analysis .......................................................... 261
7.3.10.6 Statistical analysis........................................................... 261
7.4
Accomplishments....................................................................................... 262
7.4.1
Flask studies................................................................................... 262
7.4.1.1
PAH removal ................................................................... 262
7.4.1.2
Biosurfactant production............................................... 266
7.4.1.3
Nutrient amendment ..................................................... 267
7.4.1.4
Vermiculite carrier technology ..................................... 268
7.4.2
LTU pilot project ........................................................................... 270
7.4.2.1
Chemical characteristics ................................................ 270
7.4.2.2
PAH removal ................................................................... 271
7.4.2.3
Microbial characterization............................................. 273
7.4.2.4
Soil respiration ................................................................ 276
7.4.3
Trough pilot project ...................................................................... 276
7.4.3.1
Chemical characterization ............................................. 276
7.4.3.2
PAH removal ................................................................... 276
7.4.3.3
Microbial characterization............................................. 282
7.4.3.4
Metabolic analysis: trough soil respiration ................ 283
7.4.4
Comparison of LTUs and troughs.............................................. 284
7.5
Conclusions on utility in remediation.................................................... 285
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