Environmental Engineering Reference
In-Depth Information
Construction of pRT1, pRO41, and pOCC1
PISIHIII
oriT
oriV
KpnI
pE43
ohbABCR
pE43
pSP329
8.2 kb
bhr
P
lac
trfA
pRT1
10.8 kb
G+/G
tetR
KpnI + XbaI
−
tetA
XbaI
ohbRohbABC
3.5 kb
XbaI +
PstI
P
lac
BamHI
KpnI + XbaI
XIBIMIKICIRI
pRC, 2.6 kb
XbaI
BamHI
PISIHIII
pRC
PISIHIII
pRO41
14.3 kb
G+/G −
pK4
5.2 kb
pOCC1
18.6 kb
G+/G
−
BamHI
P
lac
P
la
c
ohbRABC
XI
BamHI
ohb
RI
PstI
clc
XI
KICIRI
EcoRI
KICIRI
4.3 EcoRI-fragment
clc
(pDC100)
