Environmental Engineering Reference
In-Depth Information
chapter six
Enhancing PCB
bioremediation
James M. Tiedje, Tamara V. Tsoi, Kurt D. Pennell,
Lance D. Hansen, Altaf Wani, and Desirée P. Howell
Contents
6.1
Project background and rationale ........................................................... 148
6.2
Objectives .................................................................................................... 151
6.2.1
Overall objectives .......................................................................... 151
6.2.2
Research objectives to design PCB-growing GEMs ................ 152
6.2.3
Research objectives to enhance PCB remediation ................... 153
6.2.4
Field-Test Phase Objectives ......................................................... 154
6.3
Technical approach .................................................................................... 155
6.3.1
Summary......................................................................................... 155
6.3.2
FeSO
4
amendment......................................................................... 157
6.3.3
Sequential inoculations ................................................................ 157
6.3.4
Surfactant Amendments............................................................... 161
6.4
Accomplishments of the flask evaluation.............................................. 162
6.4.1
Designing and testing PCB-growing GEMs ............................. 162
6.4.1.1
Characterization of aerobic PCB metabolism by
biphenyl-degrading organisms .................................... 162
6.4.1.2
Conceptual proof of designing PCB
growth pathway.............................................................. 167
6.4.1.3
Developing gene transfer system for
G+/G- PCB-degrading bacteria................................... 170
6.4.1.4
Degradative capabilities of the recombinant
RHA1(
fcb
) ......................................................................... 171
6.4.1.5
Survival and activity of GEM RHA1(
fcb
) in
nonsterile soil .................................................................. 173
147
