Synthesis and Assembly of G Protein Dimers: Comparison of In Vitro and In Vivo Studies - GPCR Signalling Complexes: Synthesis, Assembly, Trafficking and Specificity - page 158

Fig. 9.2 Survey of Gb g complexes generated in vitro . [ 35 S]-methionine-labeled G b and FLAG-
Gg were synthesized independently with a rabbit reticulocyte lysate system and then combined at
a constant stoichiometry of 2:1 Gb :FLAG-G g. Samples were immunoprecipitated with immobi-
lized anti-FLAG antibody and analyzed by autoradiography to determine Gb /G g stoichiometry.
Gg8cone is the same as Gg9. Further details can be found in Dingus et al. ( 2005 )