Biomedical Engineering Reference
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Fig. 8.6
PhLP1 is required for Gb
2
dimer formation with Gg
1-13
.
The effects of siRNA-deple-
tion of PhLP1 (
a
) and over-expression of a dominant negative PhLP1 D 1-75 (
b
) on assembly of
Gb
2
with Gg
1-13
are shown. In Panel
a
, HEK-293 cells were treated with siRNA against PhLP1,
lamin A/C, or no siRNA as indicated and then transfected with FLAG-Gb
2
and the indicated
HA-Gg subunit cDNAs. Cells were lysed, immunoprecipitated with an anti-FLAG antibody, and
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