Chemistry Reference
In-Depth Information
1
μ
m
33nm
20nm
(i)
(i)
Nanoparticle modified DNA
Uniodified DNA
20 nm
10 nm
7 nm
5 nm
10
600
700
800
Wavelength (nm)
(ii)
900
1000
1100
1200
10
20
30
40
50
60
70
Temperature (°C)
(ii)
(c)
(d)
30nm
10nm
(i)
(i)
1x10
6
uPA
MMP-2
MMP-2 + uPA
No protease
2x10
6
3x10
6
4x10
6
5x10
6
6x10
6
400
500
600
700
Wavelength (nm)
(ii)
Wavelength (nm)
(ii)
Figure 18.1
Optical nanosensors.
(a) TEM image of core (gold)-shell (silicon) nanoparticles (i) and optical
resonances of gold shell-silica core nanoshells as a function of their core/shell ratio (ii) Adapted with permission
from [16] © 2004. (b) TEM image of gold nanoparticles modified with a polypeptide-base (i) and absorbencies of
gold nanoparticles modified or not with DNA (ii). Adapted from
[18]
© 2000 American Chemical Society.
(c) TEM image of quantum dots (QDs) (i) and two-plex detection of matrix metalloprotinease-2 (MMP-2), 1
m
g
ml
−1
and/or urokinase-type plasminogen activator (uPA) 10
g ml
−1
via the bioluminescence resonance energy
transfer (BRET) sensitized photoluminescence (PL) of QDs with peak emission at 655 and 705 nm, respectively
(ii). Adapted from [23] with kind permission from Springer Science
m
Business Media © 2010. (d) TEM images of
hybrid nanoparticles (i), absorbance of the binding of the enzyme to the particles sterically (b) [27] Adapted by
permission of The Royal Society of Chemistry © 2010.
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