Chemistry Reference
In-Depth Information
(a)
Detector
Column
1
6
2
Injector
D
5
3
4
Pump B
Pump C
Pump A
Waste
Solvent B
Solvent C
Solvent A
(b)
Detector
Column
1
6
2
Injector
D
5
3
4
Pump B
Pump C
Pump A
Waste
Solvent B
Solvent C
Solvent A
Figure 10.5
The diagram of switching valve for the nano LC system: (a), load and concentrate sample on
trapping column; (b), elute sample from trapping column to analytical column. D-desalting column. Solvent A is
usually a low percentage of organic solvent, Solvents B and C are used for creating gradient mobile phase. For
the green LC purpose, isocratic mode is recommended in order to replace the gradient mode.
This simple way could approximately calculate the desired flow rate or column diameter at the similar
retention time. Trial and error is also necessary to gain the most suitable LC conditions.
10.3.5
Homemade micro-scale analytical system
It is very easy to create a homemade micro-scale system from a traditional LC system. A homemade micro-
scale system is very suitable for sample separation by isocratic mode. The user can slow down the flow rate
