Chemistry Reference
In-Depth Information
Modifier-selection
valve
MeOH
2PrOH
2PrOH+
0.5% IPA
MeOH+
0.5% IPA
MeOH+
0.5% TFA
2PrOH+
0.5% TFA
CO
2
tank
Dual pump
Autosampler
Column-selection
valve
AD
OD
OJ
AS
Oven
UV detector
Waste
Figure 10.2
Chiral column- and modifier-switching system used in the SFC screen
[42]
. IPA, PrOH and TFA are
isopropylamine, isopropanol and trifluoroacetic acid. AD, OD, OJ and AS are four chiral columns.
analytical-scale pump: microbore, capillary and nano LC. These instruments could help us to reduce the
production of organic waste. Their approximate column inner diameter and pump flow rate are shown in
Table 10.2.
10.3.1
Microbore Liquid Chromatography (microbore LC)
Microbore LC is a simple alternative approach for traditional LC. Compared with traditional LC, the inner
diameter of these columns used in microbore equipment is about 1 mm (or <1 mm). The pump flow rate of
microbore LC is about 100 ~ 500
l min
−1
. All the tubing system in microbore LC is also smaller than the
traditional LC system. Microbore LC uses low flow rate and narrow tubing; this design could reduce the
usage of organic solvents and increase the sensitivity simultaneously. The same concentration of sample in
μ
