Biomedical Engineering Reference
In-Depth Information
- cells (lines and primary): human and mouse fibroblasts, human
and mouse osteoblasts, epithelial corneal cells (collaboration Pr JM
Legeais);
- bacteria involved in foreign body infections (commercial strains,
model strains and clinically isolated strains - collaboration
Pr P Vaudaux, Pr AC Crémieux:
Staphylococcus aureus
(SA),
methicillin-resistant
Staphyloccus aureus
(MRSA),
Staphylococcus
epidermidis
(SE).
During the entirety of this period, the experiments were carried out
in vitro
and had the aim of highlighting [ELK 02, BER 02, LAT 03,
LEG 03, EVA 04, LEG 05] the concept of “bioactive polymer” or
“bioactive surface”.
Among the biological properties of interest of these “bioactive
polymers”, we have shown the following:
a)
The modulation of the cell proliferation of fibroblasts and
osteoblasts
: during the radical synthesis of copolymers and by varying
the proportion of NaSS, MA and MMA monomers, it is possible to
obtain functionalized polymer films capable of modulating the
proliferation of cells and also controlling cell differentiation
[ELK 02, ANA 06].
The mechanisms which form the basis for these activities have
been highlighted and come from the selectivity of the adsorption of
binding proteins such as Fn and its structure on functionalized
surfaces which is modulated according to the chemical composition of
the polymer in functional groups SO
3
-
and COO
-
: it has been shown
that it is the differences in exposure of Fn domains to the
1
integrins of cells which leads to differences in cell signaling and
different proliferations [LAT 03, EVA 04]. It is worthy of note that
these properties are visible from the cell adhesion phase - differences
in cell adhesion strength, increased focal adhesion points [LAT 03,
LEG 05].
α
β
5
