A History of Iron Metabolism in the Mycobacteria - Iron Acquisition by the Genus Mycobacterium - page 8

Fig. 2.2 Colin Ratledge
(1936-)

Table 2.1 Metabolic consequences of iron deficiency in mycobacteria
Consequences of deficiency References

Long forms of cells produced; DNA synthesis declines Winder and O'Hara [ 8 ]

Increased activities of DNA repairing enzymes Winder and Coughlan [ 12 , 13 ]

Winder and McNulty [ 14 ]
Winder and Barber [ 17 ]

Activity decrease of iron-containing enzymes Winder and O'Hara [ 9 ]

Low content of cytochromes a and b with low content
of coproporphyrin

McCready and Ratledge [ 20 ]

Increased biosynthesis of iron chelating compounds.
• salicylic acid

Ratledge and Winder [ 50 ]

• mycobactins Snow [ 26 ]

• exochelins and carboxymycobactins Macham and Ratledge [ 88 ]

Macham et al. [ 88 , 89 ]

inadequate basis on which to conduct the subsequent studies. The procedure used,
which was derived from the work of Donald et al. [ 10 ], was to autoclave 2 L of
medium in a 5 L flask with 1 % (w/v) high-grade, activated alumina. When the
flask came out of the autoclave, the contents was immediately shaken thoroughly
and then, when cool, filtered through Whatman number 542 filter paper which was