Biology Reference
In-Depth Information
FIGURE 13.18
Two types of Amicon concentration filter devices. (a) high pressure is applied by nitrogen gas
from the top.
Courtesy of Artisan Scientific Corporation.
(b) centrifugation drives the buffer through the filter pores.
Courtesy of EMD Millipore Corporation
used for drug delivery. The sequestered volume can vary tremendously from one type of
liposome to another. Although MLVs are large, their available interior volumes are surpris-
ingly small, due to the sequestered multi-lipid bilayers.
Available sequestered
volume
Liposome type
MLV
1.8
m
g/nmol lipid
m
SUV (sonication)
0.8
g/nmol lipid
LUV (ether vaporization)
14.0
m
g/nmol lipid
E. MEMBRANE RECONSTITUTION
Previously in this chapter it was discussed how integral membrane proteins can be iso-
lated into detergent micelles, while maintaining their correct (active) conformation. It was
also shown that membrane polar lipids can be isolated and dissolved into detergent micelles,
