Biology Reference
In-Depth Information
TABLE 13.2
Visualization Methods For Identifying Lipids on TLC Plates
[21]
.
Reagent
Reacts with Lipid Identified
Ultraviolet Light
non-specific
all lipids
H
2
SO
4
heat
carbon charring
all lipids
Iodine
double bonds
lipids with double bonds
Primuline
non-specific
all lipids
Rhodamine 6G
non-specific
all lipids
Molybdate
phosphate
all phospholipids
Malachite green
phosphate
all phospholipids
Ninhydrin
primary amines
PE, PS
Dragendorf Reagent
choline
PC
2,4-dinitrophenylhydrazine
plasmalogens
Resorcinol
sugars
gangliosides
Ferric Chloride
cholesterol
maximum
distance the
eluent
traveled
distance
the spot
traveled
Eluent
FIGURE 13.7
Determination of R
f
values
[20]
.R
f
is the distance the spot travels divided by the maximum
distance the eluent travels.
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