Biology Reference
In-Depth Information
After each step in membrane purification, tests must be run to assure that the step resulted
in purification of the target membrane and that other membranes had been excluded. To
achieve this, a large number of structural markers and marker enzymes characteristic of
a particular membrane are employed.
Membrane reconstitution is often a necessary step in determining a membrane protein's
mechanism of action. Chapter 13 will review the methodologies to purify membrane proteins
and lipids and to reconstitute these components into a functional liposome. Lipid raft isola-
tion will also be discussed.
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