Biology Reference
In-Depth Information
Densities of some major biomolecules (g/ml)
Lipids
0.9
e
1.1
Proteins
1.25
DNA
1.7
RNA
2.0
In
Figure 12.10
, particle density (g/ml) is plotted against sedimentation coefficient in
Svedberg Units (
S
)
[28]
. Clearly all membrane vesicle fractions (microsomes) are close
together, making them very difficult to separate from one another. DNA and RNA, on the
other hand, are much denser than the membrane fractions and can be easily separated on
CsCl gradients.
Lysosomes
Mitochondria
Smooth ER
1.1
1.2
1.3
1.4
1.5
1.6
1.7
1.8
1.9
2.0
2.1
10
0
Plasma membrane
Chloroplasts
Rough ER
Peroxisomes
Nuclei
Soluble proteins
DNA
RNA
10
1
10
2
10
3
Sedimentation coefficient (Svedbergs)
10
4
10
5
10
6
10
7
10
8
FIGURE 12.10
Particle density in g/ml is plotted against the log of the sedimentation coefficient in Svedberg
Units. The values for DNA and RNA were determined from CsCl gradients
[28]
.
OH
OH
HO
NH
O
OH
O
NH
I
I
I
I
OH
OH
NH
HO
NH
OH
N
N
I
O
OH
I
O
O
O
CH
3
H
3
C
FIGURE 12.11
Iodixanol.
Courtesy of Focus Technology Co., Ltd.
