Biology Reference
In-Depth Information
FIGURE 9.19
Determining flip-flop rates for the primary amine phospholipids PE and PS by use of the primary
amine labels, TNBS and IAI.
PE and PS will be labeled since the reagent does not cross the membrane. The un-reacted
TNBS is then washed away from the TNBS-modified vesicles and the vesicles are allowed
to incubate for a time (t
X). During this time, some un-modified (inner leaflet) PE and
PS can flip to the outer leaflet while roughly an equivalent amount of PE-TNBS and PS-TNBS
ΒΌ
