Biology Reference
In-Depth Information
CHAPTER
9
Basic Membrane Properties of the
Fl
uid Mosaic Mod
el
OUTLINE
A. Size and Time Domains
132
E. Lipid Trans-membrane Diffusion
(Flip-Flop)
151
B. Membrane Thickness
134
Chemical Method to Determine Flip-Flop
Rates
Planar Bimolecular Lipid Membranes
134
152
X-Ray Diffraction
136
Phospholipid Exchange Protein Method
to Determine Flip-Flop Rates
C. Membrane Asymmetry
137
154
Protein Asymmetry
137
Flippase, Floppase, and Scramblase
156
Carbohydrate Asymmetry
139
Some General Conclusions from
Flip-Flop Studies
Lectins
139
157
Lipid Asymmetry
139
F. Lipid Melting Behavior
157
Chemical Modification
140
Differential Scanning Calorimetry
159
Enzymatic Modification
141
Fourier Transform Infrared (FT-IR)
Spectroscopy
Erythrocyte Lipid Asymmetry
143
163
D. Lateral Diffusion
144
Fluorescence Polarization
164
The Frye-Edidin Experiment
145
G. Membrane 'Fluidity'
170
Fluorescence Recovery after
Photobleaching (FRAP)
145
Summary
172
Single Particle Tracking (SPT)
148
References
172
Membrane Lateral Diffusion Rates:
Conclusions
150
