Biomedical Engineering Reference
In-Depth Information
Fig. 10. GFAP immunoreactivity 4 week after N-vinyl pyrrolidone exposure. GFAP 40X
Monomer 1st week 2nd week 4th week 6 th week 12 th week
Ethylene glycol ++ ++ +++ ++ ++
N-vinyl pyrrolidone +++ +++ +++ +++ ++
2-hydroxyethyl methacrylate + ++ +++ ++ ++
Acrylamide ++ ++
Methacrylamide +++ +++ ++ ++ +
N-isopropylacrylamide +++ +++ +++ +++
Acrylic acid + ++ ++ ++
Control +++ +++ +++ +++ +++
Table 1. Demonstrates the semi-quantitative scoring findings of GFAP immunolocalization
in rat medullaspinalis following 1, 2, 4, 6 and 12 weeks of particular monomer applications
monomers cause apoptosis in neuronal cells. We therefore examined apoptosis by using
terminal deoxynucleotydil transferase dUTP nick and labelling (TUNEL) method in spinal
cord (Unver Saraydin et al., 2011).
While TUNEL positive cells has been detected rarely in the control and in the ethylen glycol
application groups, numerous TUNEL positive cells were intensively observed in the spinal
cord of the methacrylamide, acrylic acid, N-vinyl pyrrolidine, acrylamide, N-
isopropylacrylamide and 2-hydroxyethyl metacrylate application groups (Figure 11-14).
Fig. 11. TUNEL-positive apoptotic cells in the control group. TUNEL 100X
