Biomedical Engineering Reference
In-Depth Information
observed to adsorb to virtually any surface, and because FN is generally considered to be
the most important cell adhesion mediating protein in serum and consequently has been
much more studied than VN.
Table 1. Protein adsorption to the surface of the (topographically patterned) PEG-based
hydrogel; qualitative results are given for samples that were incubated with serum (100% or
10% in buffer solution) or with buffer solutions containing a mixture of the pure proteins
Vitronectin (VN) and Fibronectin (FN) in various ratios. Reproduced from: Schulte et al.,
(2011)
Macromol. Biosci.
(
in press).
Copyright 2011 John Wiley and Sons.
Fig. 10. Cell adhesion on PEG hydrogels enabled by surface topography and/or pre-
incubation with the cell adhesion-mediating protein VN. Reproduced from: Schulte et al.,
Macromol. Biosci. (in press)
. Copyright 2011 John Wiley and Sons.
In order to verify whether this small but significant amount of adsorption of VN to the PEG
surface is responsible for the observed cell adhesion on topographically patterned hydrogels
we investigated the pre-incubated hydrogels (both smooth and patterned) in cell culture.
