Biomedical Engineering Reference
In-Depth Information
acids have been suggested in order to provide non-cytotoxic tissue biomaterial and
biomaterial with reduced calcification, as it is shown in table 5. In this table, it is also
included molecules of biological importance as well as peptide sequences used to improve
cell adhesion after the fixation treatment.
Type of molecule
Effects on biomaterial
References
Macromolecules
Hyaluronic
acid -
Reduce the calcification of
glutaraldehyde-treated tissue
Ohri et al., 2004
Reduce the calcium deposition
and the cytotoxicity of
glutaraldehyde-treated
Heparin-
Lee et al., 2000
Inhibit the platelet surface
attachment and spreading and
decrease the calcification of
glutaraldehyde-treated tissue
Vasudev &
Chandy, 1999;
Park et al., 1997
Poly(ethylene
glycol)-
Enhace the adhesion and
proliferation of human
mesemchymal stem cells on
acellular tissue
RGD peptides-
Dong et al., 2009
Amino acids
Reduce the protein adsorption
and platelet adhesion of
glutaraldehyde treated tissue.
However, BP treatment with
amino acids does not
effectively prevent
calcification. Incorporation of
thiol moieties to the tissue
Jorge-Herrero et
al., 1996; Jee et
al., 2003;
Mendoza-
Novelo &
Cauich-
Rodríguez, 2009
L-arginine, L-
glutamine, L-
lysine, L-
glutamic acid,
L-cysteine -
Acids
Homocysteic
acid -
Reduce toxicity but does not
affect the stability of
glutaraldehyde-treated tissue
Stacchino et al.,
1998
Amino oleic
acid -
Inhibit the calcification of
glutaraldehyde-treated tissue
Chen et al., 1994
Table 5. Molecules grafted on crosslinked bovine perichardial tissue
Fig. 5. Schematic representation of tissue crosslinking with glutaraldehyde and chemical
coupling of L-cysteine
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