Human Dentin as Novel Biomaterial for Bone Regeneration - Biomaterials: Physics and Chemistry - page 133

which have gained the approval of Food and Drug Administration (FDA) for human use.
The ZrO 2 ceramics were fabricated by sintering at 1400 ℃ for 2 h after the slip casting of the
mixture of ZrO 2 powder and distilled water (Fig. 5a). As the results of characteristics
analyses of ZrO 2 objects, the contraction rate, the relative density, and the bending strength
were 21%, 99%, and 400MPa, respectively. The automatic mill could crush a tooth and/or a
cortical bone block (1x1x1cm 3 ) under the condition of cooling using saline ice blocks (1cm 3 )
(Fig. 5b). The crushed tooth granules were decalcified completely in 0.026N HNO 3 solution
for 20 min. The DDM granules including cementum were extensively rinsed in cold distilled
water (Fig. 5e), (Murata et al., 2009; Murata et al., 2010a).
a: ZrO 2 vessel and blade, b: tooth with ice blocks, c: stainless cover, d: mill, e: DDM granules before
clinical use.
Fig. 5. Preparation of DDM using automatic tooth mill (DENTMILL®, Tokyo Iken)
Surgical procedure
Splitting osteotomy and cortical perforations were performed in the atrophied jaw and the
autogenous DDM were transplanted to the treated bone in 2006 (Fig. 6a,b,c). At 4 months
after the operation, 3 same fixtures (Synchro-steppted screw type: diameter; 3.4mm, length;
11mm, FLIALIT-2® , FRIADENT) were implanted into the augmented bone under local
anesthesia (Fig. 6b). At the same time, bone biopsy was carried out for the tissue
observation.