In vivo imaging of graftversus-host disease and graftversus-leukemia - Immune Biology of Allogeneic Hematopoietic Stem Cell Transplantation - page 62

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FIGURE 4.1
Visualization of GVHD and GVL. (A) Bioluminescence imaging of T-cell infiltration of lymphoid organs followed by migration to GVHD target tissues (adapted from Beilhack
et al. [23] ). (B) In vivo fluorescence imaging of eGFP + allogeneic splenocytes in lymph nodes and Peyer's patches 7 days after infusion (adapted from Panoskaltsis-Mortari
et al. [86] ). (C) PET imaging of FDG uptake in the colon during GVHD corresponds with tissue infiltration of eGFP + donor lymphocytes (adapted from Stelljes et al. [102] ).
(D) Magnetic resonance imaging time course of CD8 + T-cell homing to tumors. Top: axial slices through mouse thighs at (from left to right) 0 h, 12 h, 16 h and 36 h post
transfer. Bottom: three-dimensional color-scaled reconstructions of images above (adapted from Kircher et al. [113] ). (E) Bioluminescence tomography superimposed on
computed tomography (CT) data clearly visualizes T-cell precursor infiltration of the thymus (adapted from Na et al. [116] ) .