Chemistry Reference
In-Depth Information
12
100
90
10
80
70
8
60
6
50
40
4
30
20
2
10
0
0.1
1
10
100
1000
10000.0
DDMC/PTX complex
Particle size (nm)
Fig. 6.41
Particle size distribution and zeta potential of a DDMC/PTX complex were measured
by using dynamic light scattering and particle electrophoretic mobility (the SZ-100 nanopartica
series instruments from HORIBA, Ltd.)
UV absorbance vis the paclitaxel conc at 227nm.
18
16
14
12
10
8
6
4
2
0
DDMC/PTX e 62.4x10^4
(H2O)
PTX e 3.27x10^4
(acetone:H2O =50:1)
PTX e 2.8x10^4
(CH3CN:H2O =4:1)
PTX e 2.6x10^4
(MeOH:H2O =1:1)
0
0.2
0.4
0.6
Absorbance
Fig. 6.42
Absorbance of the DDMC/PTX complex. Absorbance of the DDMC/PTX complex
solution measured at a wavelength of 227 nm and light path of 10 mm
In this method, cells were plated onto 96-well tissue culture plates at a density of
10,000 cells/well to ensure that the optimal population density was reached within
24-48 h. Next, the indicated concentrations of DDMC/PTX or paclitaxel alone
were added to each well, to a final volume of 0.1 mL. The culture medium
contained up to 10 % fetal bovine serum. Cell Counting Kit-8 (CCK-8; Dojindo
Molecular Technologies, Inc.) solution was added to each well and the plate was
incubated under CO
2
at 37
C for 24 or 48 h. After adding 10
L of CCK-8 solution
to each well, the plate was gently mixed and incubated under CO
2
at 37
C for
2-4 h. The optical density of each well was measured at 450 nm and cell survival
was calculated using the following formula:
μ
