Chemistry Reference
In-Depth Information
Fig. 6.33
Effect of
cytotoxicity by DEAE-
dextran-MMA graft
copolymers at different
concentrations on transfection
of a monolayer of HEK293
cells
As shown in Figs.
6.29
and
6.31
transfection activity was determined using the
X-gal staining method and a higher value of five times or more was confirmed for
samples of DDMC1 and DDMC2 than for the starting DEAE-dextran hydrochlo-
ride. From the results, the transfection efficiency and the reaction rate of formation
of the complex should increase when using DDMC hydrochloride instead of
DEAE-dextran hydrochloride.
Cytotoxicity for the Transfection
Figure
6.33
shows the change in transfection efficiency when using twice the
protocol quantities of DDMC (for example 20
g DNA) and of both DNA and
DDMC. Transfection of HEK293 by samples DDMC1 and DDMC2 was carried out
using twice the protocol quantities of both DNA and DDMC . Transfection effi-
ciency was twice as high than with the protocol amounts, as determined using the
X-gal staining method. From these results (impossible for DEAE-dextran), cyto-
toxicity for the transfection should be confirmed to decrease and improve when
using DDMC hydrochloride instead of DEAE-dextran hydrochloride.
μ
6.4.6 Kinetics for the Transfection by DDMC
6.4.6.1 Complex Formation Reaction Mechanisms
The difference in protein expression due to DDMC and DEAE-dextran is thought to
be caused by different complex formation reactions, particularly when their
concentrations are very low. With the DNA and DDMC complex formation
reactions,
the hydrophobic bonding force is
strongly influenced by the
