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polymerase extension) yields a blunt string (with extremal primers) that is a seed of
exponential amplification.
The phenomenon of the blunt form of PCR exponential amplification is empirically
well-known, but it is interesting that it is a mathematical consequence of the com-
binatorial mechanism on which PCR is based. The following corollary is a direct
consequence of PCR lemma.
Corollary 2.2. In the PCR process of lemma above where an exponential amplifi-
cation occurs, then, at most at the third step of the process, a blunt string appears
which is a seed of an exponential amplification.
Fig. 2.37 PCR with two internal primers, where curved lines represent pairing
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