Capsid Modification Strategies for Detargeting Adenoviral Vectors - Adenovirus: Methods and Protocols - page 48

Fig. 1 Overview of strategy to produce capsid modifi ed adenoviral vectors

Ultimately, these vectors should enable the production of safer,
more effi cacious vectors for in vivo applications whose tropism can
be tailored towards individual applications.

2 Materials

2.1 Generation of
Adenoviral Vectors
with Altered Penton
Base and Hexon
Proteins

1. Proof Reading Taq polymerase (Herculase II Fusion DNA
Polymerase, #600677-41, Stratagene, CA, USA).
2. Rapid DNA Ligation kit (#11635379001, Roche, Mannheim,
Germany).
3. StrataClone Blunt PCR cloning kit (Stratagene, #240207, CA,
USA).
4. Annealing buffer: 10 mM Tris-HCl, pH 7.5-8.0, 60 mM
NaCl, 1 mM EDTA.
5. DH5

2.1.1 General Molecular
Biology Reagents

TOP10 competent bacteria.
6. 1 kB and 100 bp molecular weight ladders.

α