Biology Reference
In-Depth Information
3.2.4
Titer Calculation
To determine virus infectious titer the following equation (Eq.
1
)
must be used:
% GFP positive cells
[
TiteripmL
](. ./
)
=
×
ViralDilution
Volume of viralsuspensi
on
(1)
×
Cell concentrationatinfection
3.3 Production
in STB
The protocols described below have been designed to produce 5 L
of HAdV and 2 L CAV-2 using Biostat DCU-3 Bioreactors
(
see
Note 5
). The main points of bioreactor preparation are com-
mon for both cell culture systems used and are described in
Subheading
3.3.1
. The specific details for HAdV and CAV-2 are
described in Subheadings
3.3.2
and
3.3.3
, respectively. In Fig.
2
it
is presented a process flow chart to help in the implementation of
Fig.
2
Process flowchart
