Biology Reference
In-Depth Information
23. These reconstitution times are given for Ad5-based constructs.
Reconstitution of Ads from other species may need longer res-
cue times (5-6 days each step).
Acknowledgments
The authors would like to thank Sigrid Seelmeir and Simone Boos
for their excellent technical support during establishment and devel-
opment of these methodologies. We also would like to thank Harald
Wodrich and Ruben Martinez (University of Bordeaux) for their
generous input in testing and improvement of these techniques.
References
1. Ruzsics Z, Wagner M, Osterlehner A, Cook J,
Koszinowski U, Burgert HG (2006)
Transposon-assisted cloning and traceless
mutagenesis of adenoviruses: development of a
novel vector based on species D. J Virol
80:8100-8113
2. Ruzsics Z, Koszinowski UH (2008)
Mutagenesis of the cytomegalovirus genome.
Curr Top Microbiol Immunol 325:41-61
3. Sirena D, Ruzsics Z, Schaffner W, Greber UF,
Hemmi S (2005) The nucleotide sequence
and a fi rst generation gene transfer vector of
species B human adenovirus serotype 3.
Virology 343:283-298
4. Imelli N, Ruzsics Z, Puntener D, Gastaldelli
M, Greber UF (2009) Genetic reconstitution
of the human adenovirus type 2 temperature-
sensitive 1 mutant defective in endosomal
escape. Virol J 6:174
5. Puntener D, Engelke MF, Ruzsics Z, Strunze
S, Wilhelm C, Greber UF (2011) Stepwise loss
of fl uorescent core protein V from human ade-
novirus during entry into cells. J Virol
85:481-496
6. Wodrich H, Henaff D, Jammart B, Segura-
Morales C, Seelmeir S, Coux O et al (2010) A
capsid-encoded PPxY-motif facilitates adeno-
virus entry. PLoS Pathog 6:e1000808
7. Jager L, Hausl MA, Rauschhuber C, Wolf
NM, Kay MA, Ehrhardt A (2009) A rapid pro-
tocol for construction and production of high-
capacity adenoviral vectors. Nat Protoc
4:547-564
8. Warming S, Costantino N, Court DL, Jenkins
NA, Copeland NG (2005) Simple and highly
effi cient BAC recombineering using galK
selection. Nucleic Acids Res 33:e36
9. Alper MD, Ames BN (1975) Positive selection
of mutants with deletions of the gal-chl region
of the Salmonella chromosome as a screening
procedure for mutagens that cause deletions.
J Bacteriol 121:259-266
10. Lemnitzer F, Raschbichler V, Kolodziejczak D,
Israel L, Imhof A, Bailer SM et al (2013)
Mouse cytomegalovirus egress protein pM50
interacts with cellular endophilin-A2. Cell
Microbiol 15:335-351
11. Bubeck A, Wagner M, Ruzsics Z, Lotzerich M,
Iglesias M, Singh IR et al (2004)
Comprehensive mutational analysis of a her-
pesvirus gene in the viral genome context
reveals a region essential for virus replication.
J Virol 78:8026-8035
12. Wagner M, Koszinowski UH (2004)
Mutagenesis of viral BACs with linear PCR
fragments (ET recombination). Methods Mol
Biol 256:257-268
13. Cherepanov PP, Wackernagel W (1995) Gene
disruption in Escherichia coli: TcR and KmR
cassettes with the option of Flp-catalyzed exci-
sion of the antibiotic-resistance determinant.
Gene 158:9-14
14. Sambrook J, Russell DW (2001) Molecular
cloning: a laboratory manual, 3rd edn. Colb
Spring Harbor LAboratory Press, Cold Spring
Harbor, New York
15. Stalker DM, Kolter R, Helinski DR (1979)
Nucleotide sequence of the region of an origin
of replication of the antibiotic resistance plasmid
R6K. Proc Natl Acad Sci U S A 76:1150-1154
