Biology Reference
In-Depth Information
8. Pre-wetting the ELISptot plate is an optional step to improve
antibody binding, sensibility, and quality of spots. Nitrocellulose
plates should not be treated with ethanol, only PVDF plates.
MSIP PDVF plates should be treated with 15
L of 35 % etha-
nol per well for 1 min. MAIPSWU PDVF plates should be
treated with 50
μ
μ
L of 70 % ethanol per well for 2 min.
Acknowledgments
This work was supported by a grant from the Spanish Ministry of
Education and Science BIO2011-30299-C02-01, BIO2008-
04692-C03-01 and from the Generalitat de Catalunya
2009SGR1212.
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