Biology Reference
In-Depth Information
Fig. 1
HCMV secretome mediates EC tubule formation.
a
Quantitation of two parameters of
angiogenesis, lumen formation, and number of branch points at 24 h after plating on matrigel in
the presence of control supernatants (SFM alone or SFM + HS/ECGS) and test supernatants con-
ditioned by factors secreted by Mock- and HCMV-infected cells.
b
Low-power images of EC dif-
ferentiation on matrigel.
c
High-power images, conditions as for (
b
), illustrating the integrity of
individual tubules.
d
Tubule survival after 2 weeks on matrigel in the presence of SFM plus HS/
ECGS or supernatant conditioned by HCMV-infected cells
monolayer is reflected by a marked increase in impedance, since cells restrict the
effective area available for current flow. Fluctuations in measured impedance
occur in response to micromotions of cells and can be used as indications of cell
viability or morphology change (Keese et al. 2004). ECIS technology has been
adapted for wound healing assays (Charrier et al. 2005). Cells are grown to con-
fluence on the arrays to achieve high impedance values and a transient voltage
spike is applied to kill only the cells on the electrode. Normal ECIS measure-
ments are then used to monitor of the rate of repopulation of the wounded area
from cells surrounding the electrode, which is superior to traditional WH assays
