Biology Reference
In-Depth Information
We also assayed for changes in 174 common cytokines/growth factors present in
the HCMV secretome using RayBio
®
Human Cytokine Array G Series 2000 anti-
body arrays. We analyzed secretomes from HCMV- and mock-infected fibroblasts.
We detected 144 of the 174 factors in the HCMV secretome when our cut-off value
Table 2
Detection of factors present in the HCMV and mock secretomes by Raybiotech Protein
Array Analysis
Induced or repressed factors
Most abundant factors in
HCMV-
HCMV secretome
Mock
infected
Fold
Average
Protein
average
average
change
Protein
intensity
GDNF
25±6
5666±814*
225.3
IL-5
24633
GM-CSF
28±5
566±813*
199.4
IL-6
24618
MIP-1 alpha
336±117
22519±2926*
66.9
MIP-1 alpha
22519
MIP-3 alpha
17±12
896±92*
51.5
PECAM-1
20638
MIF
311±129
15732±2729*
50.6
PIGF
18107
MIP-1delta
26±13
915±104*
35.5
GRO
17656
GRO
614±143
17656±4136*
28.8
GITR-Ligand
17469
GITR-Ligand
655±209
17469±4198*
26.7
MIF
15732
IL-5
1037±443
24633±2638*
23.8
VE-Cadherin
15200
IL-6
1037±447
24617±2641*
23.7
TIMP-4
15136
MIP-1 beta
435±89
7058±238*
16.2
PDGF-AA
11622
RANTES
39±6
596±90*
15.2
PDGF-AB
11595
Angiogenin
87±53
676±47*
7.7
TIMP-1
8920
IL-8
234±162
1787±400*
7.6
PDGF-BB
8880
ITAC
251±189
1115±227*
4.4
TIMP-2
8839
Advin A
308±119
1290±229*
4.2
MIP-1 beta
7058
ALCAM
282±130
1181±264*
4.2
GDNF
5666
MCP-3
26±9
102±22*
4.0
GM-CSF
5661
MMP-9
35±14
113±39
3.3
TNF-RI
3574
MCP-4
35±10
111±24*
3.2
TNF RII
3451
IL-1alpha
123±38
372±48*
3.0
HCC-4
3193
IL-6 R
266±92
770±297
2.9
MCP-1
3186
IL-1beta
35±2
102±18*
2.9
LIGHT
3185
IGF-II
85±22
241±95
2.8
HGF
3174
TNF RII
1316±959
3451±867*
2.6
Oncostatin M
2724
bFGF
195±104
503±70*
2.6
Osteoprotegerin
2710
FGF-7
196±33
485±12*
2.5
IL-8
1787
Fit-3 Ligand
191±23
468±13*
2.4
EGF-R
1773
IL-16
130±35
307±36*
2.4
egp 130
1732
MCP-1
1388±926
3186±414
2.3
Advin A
1290
AgRP
181±88
416±68*
2.3
TRAIL R3
1266
LIGHT
1394±934
3185±411
2.3
Thrombopoietin
1221
ICAM-1
325±185
725±267
2.2
IL-1R II
1210
IL-9
271±62
559±122*
2.1
ALCAM
1181
TGF-alpha
220±137
433±42
2.0
ICAM-2
1123
IP-10
531±77
975±105*
1.8
ITAC
1115
uPAR
1109
TECK
313±126
121±4
0.4
IL-2 R alpha
1029
(continued)
