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Fig. 3
Map of HCMV miRNAs. Relative positions of HCMV miRNAs were mapped by aligning the sequence of 11 miRNAs found in the miRNA registry
with the Towne BAC sequence. The Towne BAC genome (illustrated in the
middle
) consists of two unique regions bracketed by repeat regions. Regions of
the genome containing miRNAs have been expanded. The Towne BAC is missing ORFs US1-11 and UL150. To accommodate HCMV miRNAs in these
regions, we have used the HCMV Merlin sequence, which possesses a similar arrangement of ORFs as Towne BAC. Growth phenotype of deletion mutants
is indicated for ORFs that either contain or immediately surround HCMV miRNAs. Scale in kilobase pairs
