Human Cytomegalovirus Genome - Human Cytomegalovirus - page 8

newORF1 newORF1 newORF1

UL14 UL14 UL14

UL15a UL15a UL15a

UL17 UL17 UL17

RL1 RL2* RL3* RL4 RL5 RL6 RL6 RL7* RL9* RL10 RL12:UL154 UL1 UL3 UL3 UL3 UL4 UL4 UL4 UL5 UL5 UL5 UL6 UL6 UL6 UL7 UL7 UL7 UL8 UL8 UL8 UL9 UL9 UL9 UL10 UL10 UL10 UL11 UL11 UL11 UL13 UL13 UL13 UL16 UL16 UL16

miR-UL22A-1 miR-UL22A-1 miR-UL22A-1

miR-UL22A-1* miR-UL22A-1* miR-UL22A-1*

UL22*
UL21a

UL22*
UL21a

UL22*
UL21a

C-ORF8 C-ORF8 C-ORF8

C-ORF7 C-ORF7 C-ORF7 C-ORF9 C-ORF9 C-ORF9 newORF2 newORF2 newORF2

UL21.5 UL21.5 UL21.5 UL26 UL26 UL26 C-ORF10 C-ORF10 C-ORF10 C-ORF11 C-ORF11 C-ORF11

UL18 UL18 UL18 UL19 UL19 UL19 UL20 UL20 UL20 UL21 UL21 UL21 UL23 UL23 UL23 UL24 UL24 UL24 UL25 UL25 UL25 UL27 UL27 UL27 UL30 UL30 UL30 UL31 UL31 UL31 UL32 UL32 UL32 UL33 UL33 UL33 UL34 UL34 UL34 UL35 UL35 UL35

UL28 / 29

miR-UL22-1 miR-UL22-1 miR-UL22-1 miR-UL31-1 miR-UL31-1 miR-UL31-1

C-ORF13 C-ORF13 C-ORF13

UL41* UL41* UL41*

C-ORF12 C-ORF12 C-ORF12 C-ORF14 C-ORF14 C-ORF14

UL37 UL37 UL37

UL48a* UL48a* UL48a*

UL36 UL36 UL36 UL38 UL38 UL38 UL40 UL40 UL40 UL42 UL42 UL42 UL43 UL43 UL43 UL44 UL44 UL44 UL45 UL45 UL45 UL46 UL46 UL46 UL47 UL47 UL47 UL48 UL48 UL48

miR-UL36-1 miR-UL36-1 miR-UL36-1

UL59 UL59 UL59 newORF3 newORF3 newORF3

UL51 UL51 UL51 UL53 UL53 UL53

UL50 UL50 UL50 UL56 UL56 UL56

UL58* UL58* UL58* UL60 UL60 UL60 UL62 UL62 UL62

UL49 UL49 UL49 UL52 UL52 UL52 UL54 UL54 UL54 UL55 UL55 UL55 UL57 UL57 UL57

miR-UL53-1 miR-UL53-1 miR-UL53-1 miR-UL54-1 miR-UL54-1 miR-UL54-1

UL82as UL82as UL82as

UL81* UL81* UL81*

miR-UL70-1 miR-UL70-1 miR-UL70-1

UL80.5 UL80.5 UL80.5

UL68* UL71 UL73 UL76 UL79

UL68* UL68* UL71 UL71 UL73 UL73 UL76 UL76 UL79 UL79

newORF5 newORF5 newORF5 UL67 UL67 UL67 UL69 UL69 UL69 UL70 UL70 UL70 UL72 UL72 UL72 UL74 UL74 UL74 UL75 UL75 UL75 UL77 UL77 UL77 UL78 UL78 UL78 UL80 UL80 UL80 UL82 UL82 UL82

UL91 UL91 UL91

newORF6 newORF6 newORF6

C-ORF15 C-ORF15 C-ORF15 UL85 UL85 UL85 UL89 UL89 UL89 UL92 UL92 UL92 UL94 UL94 UL94 UL89 UL89 UL89

UL96 UL96 UL96

UL83 UL83 UL83 UL84 UL84 UL84 UL86 UL86 UL86 UL87 UL87 UL87 UL88 UL88 UL88 UL93 UL93 UL93 UL95 UL95 UL95 UL97 UL97 UL97

miR-UL111a-1 miR-UL111a-1 miR-UL111a-1

UL111 UL111 UL111 C-ORF18 C-ORF18 C-ORF18

C-ORF16 C-ORF16 C-ORF16

miR-UL112-1 miR-UL112-1 miR-UL112-1

newORF7 newORF7 newORF7 UL103 UL103 UL103

miR-UL102-1 miR-UL102-1 miR-UL102-1

C-ORF19 C-ORF19 C-ORF19

UL107 UL107 UL107 C-ORF17 C-ORF17 C-ORF17 UL111a UL111a UL111a UL114 UL114 UL114 C-ORF20 C-ORF20 C-ORF20

UL99 UL99 UL99 UL101* UL101* UL101* UL104 UL104 UL104

UL112 / 113

UL100 UL100 UL100 UL108* UL108* UL108* UL110* UL110* UL110* UL115 UL115 UL115 UL116 UL116 UL116 UL117 UL117 UL117

UL98 UL98 UL98 UL102 UL102 UL102 UL105 UL105 UL105

miR-UL102-2 miR-UL102-2 miR-UL102-2

C-ORF25 C-ORF25 C-ORF25

UL128* UL128* UL128*

UL126 UL126 UL126 newORF11 newORF11 newORF11

C-ORF22 C-ORF22 C-ORF22 newORF10 newORF10 newORF10 UL141 UL141 UL141

C-ORF23 C-ORF23 C-ORF23

UL137* UL137* UL137* C-ORF26 C-ORF26 C-ORF26

UL125* UL125* UL125* UL146:UL152 UL146:UL152 UL146:UL152

UL131 UL131 UL131

C-ORF21 C-ORF21 C-ORF21 UL121 UL121 UL121 newORF9 newORF9 newORF9 UL129* UL129* UL129* IRL14* IRL14* IRL14* C-ORF24 C-ORF24 C-ORF24 UL134* UL134* UL134*

UL145 UL145 UL145 UL139 UL139 UL139 UL138 UL138 UL138

UL124 UL124 UL124

UL118/119 UL122 UL122 UL122 UL123 UL123 UL123 UL127 UL127 UL127 UL130 UL130 UL130 UL132 UL132 UL132 UL148 UL148 UL148 UL147 UL147 UL147 UL144 UL144 UL144 UL142 UL142 UL142 UL141 UL141 UL141 UL140 UL140 UL140 UL136 UL136 UL136 UL135 UL135 UL135 UL133 UL133 UL133 UL148b UL148b UL148b

UL120 UL120 UL120

miR-US5-1 miR-US5-1 miR-US5-1

C-ORF28 C-ORF28 C-ORF28

miR-US4 miR-US4 miR-US4

UL151 UL151 UL151 US5 US5 US5 C-ORF27 C-ORF27 C-ORF27 C-ORF29 C-ORF29 C-ORF29

UL149 UL149 UL149 US21 US21 US21

miR-US5-2 miR-US5-2 miR-US5-2

UL150 UL150 UL150 IRS1 IRS1 IRS1 US1 US1 US1 US2 US2 US2 US3 US3 US3 US6 US6 US6 US7 US7 US7 US8 US8 US8 US9 US9 US9 US10 US10 US10 US11 US11 US11 US12 US12 US12 US13 US13 US13 US14 US14 US14 US15 US15 US15 US16 US16 US16 US17 US17 US17 US18 US18 US18 US19 US19 US19 US20 US20 US20

miR-US29-1 miR-US29-1 miR-US29-1

US33 US33 US33

US25* US25* US25* US30 US30 US30 US31 US31 US31

US27 US27 US27 US28 US28 US28 US32 US32 US32 US34 US34 US34

US22 US22 US22 US23 US23 US23 US24 US24 US24 US26 US26 US26 US29 US29 US29 TRS1 TRS1 TRS1

miR-US33-1 miR-US33-1 miR-US33-1

miR-US25-2-3p
miR-US25-2-5p

miR-US25-2-3p
miR-US25-2-5p

miR-US25-2-3p
miR-US25-2-5p

miR-US25-1 miR-US25-1 miR-US25-1

Fig. 2 Genomic arrangement of clinical HCMV strains. HCMV ORFs that are conserved in the five
sequenced HCMV strains (FIX, Ph, TR, Toledo and Merlin) are arranged in the conventional HCMV
map organization. ORFs are represented as arrows demonstrating the relative orientation of ORFs;
and, where applicable, black carat symbols connect exons. In several cases, e.g., UL37, UL122 and
UL123, numerous spliced variants are known, but only one abundant variant is shown on the map.
The color codes designate ORFs that are essential ( red arrows ), augmenting ( yellow arrows ) or nones-
sential ( green arrows ) for replication within cultured fibroblasts. Gray arrows represent ORFs that are
have not yet been tested for function. Red , yellow , green and gray ORFs are conserved in CCMV;
white ORFs are not conserved in CCMV. ORFs with an asterisk do not contain an AUG ≥ 80 codons
from a stop codon. The three blue boxes represent the repeat sequences found at the ends of the unique
long and unique short regions. The orange pins designate the location of virus-coded miRNAs. Their
placement above or below the sequence line designates the strand on which they are encoded. Each
tick mark on the black sequence line represents 1 kb of DNA