Functional Roles of the Human Cytomegalovirus Essential IE86 Protein - Human Cytomegalovirus - page 133

Table 1 Effect of amino acid deletions or substitutions on the functions of the IE86 protein
Effect on function Amino acids Assay Reference

Dimerization 388-542 DNA binding Ahn et al. 1998

463-513 Macias et al. 1996

Macias and Stinski 1993

Still dimerizes 551-579 Waheed et al. 1998

DNA binding 388-542 Ahn et al. 1998

346-579 Chiou et al. 1993

542-579 Jupp et al. 1993b

Macias and Stinski 1993

Still binds DNA 551-579 Macias et al. 1996

Autoregulation of MIEP 427-435 Reporter Chiou et al. 1993

505-511 Hermiston et al. 1990

H446/H452 Macias and Stinski 1993

Macias et al. 1996

Virus Petrik et al. 2007

Interaction with TBP 290-504 Protein Caswell et al. 1993

266-269 Binding Hagemeier et al. 1992a

271-275 Jupp et al. 1993a

Interaction with TFIIB 290-542 Caswell et al. 1993

Interaction with CREB 290-410 Lang et al. 1995

Transactivation 1-98 Reporter Malone et al. 1990

of promoters

25-85 Pizzorno et al. 1991

169-194 Stenberg et al. 1989

175-180 Stenberg 1996

195-579 Yeung et al. 1993

501-511

544-579

Early gene expression 258-275 Virus Heider et al. 2002

266-275

E550, E554, E558, Reporter Yeung et al. 1993

D559, D561,

E568, D573

P535,Y537 Virus Petrik et al. 2007

Late gene expression 136-290 Virus Sanchez et al. 2002

356-359 White et al. 2004

No viral growth 501-511 White et al. 2004

Delayed viral growth 258-275 Barrasa et al. 2005

266-275

Accelerated viral growth 271-275 Virus Barrasa et al. 2005

Interaction with Rb 290-390 Protein Sommer et al. 1994

Binding

Interaction with p53 325-448 Zhang et al. 2006

Interaction with mdm2 326-449 Zhang et al. 2006

Cell cycle arrest 260-279 Reporter Wiebusch and

Hagemeier 1999

451-579

Q548 Virus Petrik et al. 2006