3D PRINTING AND NANOMANUFACTURING - 3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine - page 45

FIGURE 2.14
Immunofluorescence images showing the biological functionality of the HUVEC seeded scaffolds ( Gauvin
et al. , 2012 ).
2.4.2 BIOPRINTING APPROACH TO TISSUE CONSTRUCTS
Embryonic chick spinal cord cells were patterned on untreated glass surface using LGDW in order
to fabricate small arrays of cells for the cell-cell interaction study ( Odde and Renn, 2000 ). A tunable
diode laser beam was employed at 450 mW laser power. For long-range transport of cells, the hol-
low optical fibers were coupled with a laser beam. A laser beam having 800 nm wavelength created
gradient force, which propelled cells into the center of the beam at a mean velocity of 11.4 m m/s. The
average deposition rate was 2.5 cells/min. In the hollow-fiber experiment, the cells traveled distances
of up to 7 mm.
HUVECs were guided on a Matrigel ® -coated slide to fabricate two- and three-dimensional cell
patterns using LGDW ( Nahmias et al. , 2005 ). LGDW employed a weakly focused diode laser beam