Biology Reference
In-Depth Information
Multiple/Selected Reaction Monitoring (SRM/MRM).
While writing this chapter, the guidelines for quantifi ca-
tion were being reviewed under the PSI formal document
process.
(d) MIAPE modules on molecular interaction experiments:
-
Minimum information about a molecular interaction
experiment
(MIMIx) [
35
]: it describes the minimum infor-
mation to report about molecular interaction experiments.
It includes data, such as the host organism where the inter-
action has been detected, the interaction detection
method, the list of participants in the interaction together
with their identifi ers in a public database, their biological
and experimental roles.
-
Minimum information about a protein affi nity reagent
(MIAPAR) [
36
]: it describes the protein affi nity reagent
characterization, such as antibodies used as protein identi-
fi cation tools. This includes the description of molecules
participating in the interaction—i.e., the reagent molecule
and the target molecule—features about the interaction,
such as sensitivity, selectivity, the recognized epitope,
binding constants, applications; and the characterization
of the interaction by information such as kinetic constants,
affi nity measures, or the description of the characterization
method of the reagent.
-
Minimum information about a bioactive entity
(MIABE)
[
37
]: it describes drug-target data, including information
such as molecule properties, molecule production, physi-
cochemical properties, in vitro cell-free assays, whole
organism studies and pharmacokinetic studies.
4
MIAPE Guidelines Evolution
Proteomics is a rapidly evolving fi eld. New technologies and modifi -
cations of methods are regularly appearing in the literature and on
the market that widen the range of tools available to proteomics
scientists. Better understanding of the strength and limitations of
these processes involves also the need for more precise requirements
and SOPs that target optimized quality of the obtained results, As a
consequence the MIAPE guidelines need to stay aligned with this
evolution. Also, as already mentioned, scientifi c journals have actively
participated in dedicated round tables to better align the generic
MIAPE technical reporting guidelines with their own submission
guidelines that include quality judgment aspects in addition to
descriptive data and meta-data requirements [
4
,
5
]. Representatives
from each journal have shown statistics and data about how scientists
