Biology Reference
In-Depth Information
14. Fill 50 mL syringe with cathode buffer and use needle with
diameter thinner than spacer thickness to rinse wells.
15. If a microsyringe is used, rinse with anode buffer before apply-
ing a new sample. If wells remain unused, load detergent mix-
ture 1 or 2 to the empty well.
16. Depending on laboratory condition and available equipment,
the run of the native gel electrophoresis could be done in the
cold room. In order to control the running behavior, check
temperature and pH of cathode buffer in upper buffer
chamber.
17. Gel requires about 18 h to complete separation of the solubi-
lized protein complexes (Hoefer SE400, 100 V constant, Gel
length = 160 mm).
18. The use of low fl uorescent glass plates is recommended.
References
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Analysis of molecular masses and oligomeric
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protein machines: preparing the next genera-
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8. Krause F (2006) Detection and analysis of
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