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11. Since polyubiquitinated proteins have higher molecular weight,
lower concentration polyacrylamide gels (7.5-10 %) are appro-
priate for the effi cient separation of proteins.
Acknowledgments
This work This work was supported by Grants-in-Aid for Scie
(23380198 (23380198, 24114701) to JY,
Fellowshi Fellowships from the Japan Society for the Prom
for Young for Young Scientists (2012-2014) to K.S and also by Cooper
Research Research Grant of the Gene Research Center, the University of
Tsukuba.
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