Biology Reference
In-Depth Information
Table 1
Shotgun phosphoproteomics studies in plants
Identifi ed
phosphopeptide
Phosphopeptide
enrichment
Plant material
Reference
Arabidopsis cell (membrane fraction)
283
SCX-IMAC
[
21
]
Arabidopsis cell (membrane fraction)
1,172
SCX-MOC
[
22
]
Arabidopsis cell (membrane fraction)
?
SCX-IMAC
[
23
]
Arabidopsis cell
2,597
HAMMOC
[
12
]
Arabidopsis plant
3,029
SCX-IMAC
[
24
]
SCX-MOC
Medicago root
3,457
SCX-IMAC
[
25
]
Arabidopsis cell
ca. 500
MOC
[
26
]
Rice cell
6,919
HAMMOC
[
11
]
Arabidopsis plant
4,675
HAMMOC
[
27
]
Arabidopsis plant
3,589
IMAC
[
28
]
MOC
Arabidopsis root
849
HAMMOC
[
29
]
IMAC
Soybean seed
2,001
HAMMOC
[
30
]
Rapeseed seed
IMAC
Arabidopsis seed
Medicago root
15,335
SCX-IMAC
[
31
]
Arabidopsis pollen
598
SCX-IMAC
[
32
]
SCX-MOC
Maize leaf
3,664 phosphosite
SCX-IMAC
[
33
]
Soybean root hair
1.625
Ni-NTA
[
34
]
Phosphopeptide enrichment is one of the most rapidly developing
methods in the fi eld of modifi ed proteomics. The development of
phosphopeptide enrichment methods enabled shotgun pro-
teomics-based phosphoproteomics (shotgun phosphoproteomics),
by which thousands of phosphoproteins and their modifi ed sites
could be identifi ed simultaneously (Table
1
) [
2
]. Currently, immo-
bilized metal-ion affi nity chromatography (IMAC) and metal oxide
chromatography (MOC), which are based on the affi nity of metals
for phosphate, are the most widely accepted approaches for
phosphopeptide enrichment [
3
,
4
]. Original protocols for IMAC
