Biology Reference
In-Depth Information
31. It is important to cover the entire gel apparatus with alumi-
num foil to minimize the exposure of CyDyes to light.
32. Preparative gels typically contain 500-1,000
g of protein and
are otherwise run identically to the DIGE gels. The resulting
gels are stained with colloidal Coomassie blue for visualization.
33. Dried gel pieces are lightweight and can acquire static charge;
take care to ensure that gel pieces do not “jump” out of wells.
34. Care must be taken to avoid introducing air bubbles and
touching the MALDI plate with the pipette tip.
35. We typically run a 15-min gradient at 10
μ
L/min.
36. We typically run a 60-min gradient at 300 nL/min.
μ
Acknowledgments
This work was supported by the ARC Centre of Excellence for
Plant Energy Biology (CE0561495) and A.H.M. as an ARC
Future Fellow. R.P.J. is supported by a Grains Research and
Development Corporation (GRDC) PhD scholarship.
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