Biology Reference
In-Depth Information
160
In-solution digestion (DRM)
140
120
Experiment 1
Experiment 2
Experiment 3
Experiment 4
100
80
60
40
20
0
1 2 3 4 5 6 7 8 9 101112131415161718192021222324
The number of transmembrane domains
Fig.
5
Distribution of the number of transmembrane domains in oat DRM proteins identifi ed using the “in-
solution digestion” protocol. Peptides were analyzed as described in Fig.
4
. Compared to PM (Fig.
4b
), in DRM,
more transmembrane proteins, especially those containing more than fi ve transmembrane domains, could be
identifi ed
200
180
160
140
120
Experiment 1
Experiment 2
Experiment 3
Experiment 4
100
80
60
40
20
0
In-gel digestion
In-solution digestion
Fig.
6
Estimated number of proteins with secretory signal peptides in oat PM. 173 and 81 proteins with signal
peptides were identifi ed from four replicates of in-gel or in-solution tryptic digests of 5
μ
g oat PM proteins,
respectively
