Biology Reference
In-Depth Information
a
Hot
2000
117.11385
z=1
1500
Control
1000
116.11053
z=1
500
0
115.8
116.0
116.2
116.4
116.6
116.8
117.0
117.2
117.4
m/z
b
F V V
H E LE DD LG b[+1]
G L D D
E L E H V y[+1]
350
y
10
2+
, b
10
2+
300
250
200
E
E
y
11
2+
, y
5
+
y
2
+
, y
8
3+
150
100
y
7
+
y
8
+
50
0
200
400
600
800
1000
1200
1400
1600
P]
Fig.
3
A case study of superoxide dismutase [Cu-Zn] protein in chloroplasts of rice seedlings. The chloroplast
proteins isolated from seedlings grown in hot (33 °C, 12-h light/28 °C, 12-h dark) and control (28 °C, 12-h
light/23 °C, 12-h dark) conditions were labeled with iTRAQ-116 and -117 reagents, respectively. (
a
) Quantitation
of iTRAQ-116 and -117 reporter ions for FVVHELEDDLG peptide. (
b
) MS/MS spectrum of a representative
superoxide dismutase [Cu-Zn] peptide (FVVHELEDDLG) detected in the iTRAQ labeling experiments
dicotyledons such as spinach, tobacco, potato, and
Arabidopsis
,
the starch granules, which have high density, often break
through and escape from the enveloping membrane of the
chloroplasts during centrifugation. Moreover, it is diffi cult to
prevent the contamination of starch granules during chloro-
plast preparation. It is therefore necessary to consider mea-
sures to reduce the starch content. The starch content can be
reduced by allowing the plant to grow for 2-3 days in darkness
