Biology Reference
In-Depth Information
20. A convenient time pattern can be achieved by starting a reaction
in the cuvette every 15-20 s. All additions of solutions and the
determination of the OD at 820 nm follow this time regime.
21. Depending on the protein concentration the reaction time can
be extended up to 3 h. It should be noticed that starting 60
cuvettes in a 15-s time pattern takes 15 min and therefore is
the minimum incubation time.
Acknowledgements
The work in G.O.s lab is partially fi nanced by the Austrian Science
Fund (P21298-B03) and the University Priority Programme
“Biosciences and Health.” The technical assistance of Aniela
Sommer with the Arabidopsis pollen germination is appreciated.
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