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Fig. 2
Germination of lily pollen grains in Med M. For each time point 200-300 pollen grains were counted. Five
independent repetitions, mean ± S.D.
Fig. 3
In vitro culture of
Arabidopsis thaliana
pollen in different germination media. Fresh pollen grains were
collected from fl owers in which the anthers were still shorter than the pistils and media were prepared according
to [
3
-
6
]. (
a
) Tube length after 5-h incubation in the media. Numbers indicate the number of pollen tubes
measured. (
b
) Germination frequency of
Arabidopsis
pollen grains in the given media
1. Spread
Medicago truncatula
pollen grains onto the solidifi ed
Arabidopsis germination medium described by Li et al. [
4
] and
incubate for up to 5 h. Pollen tubes can be seen after 45 min.
3.1.4 Medicago
truncatula
Pollen Culture
