Biology Reference
In-Depth Information
Some representative xylem sap proteomes
Total number
of identifi ed
proteins
a
Number of proteins
predicted to have a
signal peptide
b
Plant
Reference
Protein separation
c
/MS strategy
B. napus
[
6
]
40
39
2D-E
LC-MS/MS (ESI-Q-TOF)
B. napus
[
5
]
10
10
1D-E
LC-MS/MS (ESI-Q-TOF)
B. oleracea
[
7
]
189
164
Short 1D-E run (three samples)
LC-MS/MS (LTQ XL ion trap)
B. oleracea
[
17
]
24
14
2D-E
LC-MS/MS (LTQ-Orbitrap)
B. oleracea
[
5
]
10
10
1D-E
LC-MS/MS (ESI-Q-TOF)
Cucurbita
maxima
[
5
]
11
11
1D-E
LC-MS/MS (ESI-Q-TOF)
Cucumis
sativus
[
5
]
9
9
1D-E
LC-MS/MS (ESI-Q-TOF)
G. max
[
9
]
24
18
2D-E
LC-MS/MS (LCQ-DECA XP
Plus ion trap)
G. max
[
10
]
14
11
1D-E
LC-MS/MS (LTQ-Orbitrap)
G. max
[
22
]
17
11
2D-E
LC-MS/MS (ESI-Q-TOF)
O. sativa
[
12
]
118
71
1D-E
LC-MS/MS (ion trap)
2D-LC-MS/MS (ion trap)
P. trichocarpa
x
P. deltoides
[
13
]
97
33-24 (NC)
1D-E
LC-MS/MS (ESI-Q-TRAP)
S. lycopersicon
[
19
]
5
5
1D-E
MALDI-TOF MS
S. lycopersicon
[
20
]
20
20
2D-E
MALDI-TOF MS
LC-MS/MS (ESI-Q-TOF)
Z. mays
[
16
]
59
58-1 (NC)
2D-E
LC-MS/MS (ESI-Q-TOF)
Z. mays
[
18
] 26 22 2D-E
LC-MS/MS (ESI-Q-TOF)
a
Only non redundant proteins are considered. Numbers in bold correspond to proteins identifi ed using EST or genomic
data of the studied plant
b
Signal peptides are predicted using TargetP (
http://www.cbs.dtu.dk/services/TargetP/
) or SignalP (
http://www.cbs.
dtu.dk/services/SignalP/
)
. When available, the number of nonclassically secreted proteins (NC) determined using
SecretomeP is indicated (
http://www.cbs.dtu.dk/services/SecretomeP/
)
c
1D-E and 2D-E respectively mean one- and two-dimensional electrophoresis
