Biology Reference
In-Depth Information
Leaves
10 % TCA in acetone containing 0.07 %
2-ME
9 M urea, 1 % CHAPS, 1 % DTT, 1 %
pharmalytes pH 3-10
IPG, 2-DE, MALDI-TOF
MS, LC-MS/MS
>300
[
19
]
100 mM Tris-HCl pH 8.8, 50 mM
DTT, 10 mM EDTA, 1 mM PMSF,
1
8.5 M urea, 2.5 M thiourea, 5 %
CHAPS, 2 % Triton X-100, 100 mM
DTT, 1 % ampholytes pH 3-10
IPG, 2-DE
>1,400
[
47
]
M pepstain A, 10 mM leupeptine/
Tris-saturated phenol/100 %
methanol containing 100 mM
ammonium acetate, 10 mM DTT
μ
0.5 M Tris-HCl (pH 8.3), 2 % NP-40,
20 mM MgCl
2
, 2 % 2-ME, 1 mM
PMSF, 0.7 M sucrose/Tris-HCl
saturated phenol (pH 8.0)/cold
methanol containing 0.1 M
ammonium acetate for precipitation
8.5 M urea, 2.5 M thiourea, 5 %
CHAPS hydrate, 1 % DTT, 1 % Triton
X-100, and 0.5 % ampholytes pH
3-10/5-8
IEF tube gel, 2-DE,
MALDI-TOF MS
>500
[
24
]
Mature seeds
50 % phenol, 0.45 M sucrose, 5 mM
EDTA, 0.2 % 2-ME, 50 mM Tris-
HCl pH 8.8
8 M urea, 2 M thiourea, 2 % CHAPS,
2 % Triton X-100, 50 mM DTT,
2 mM TBP, 0.5 % ampholytes
IPG, 2-DE, MALDI-TOF
MS
≥
128
[
22
]
10 % TCA in acetone containing
9 M urea, 1 % CHAPS, 1 % DTT, 1 %
ampholytes pH 3-10
IPG, 2-DE, MALDI-TOF
MS, LC-MS/MS
<50
[
20
]
0.07 % 2-ME
50 mM Tris-HCl pH 8.8, 1.5 mM KCl,
10 mM DTT, 1.0 mM PMSF, 0.1 %
SDS/0.1 M ammonium acetate in
methanol for precipitation
7 M urea, 2 M thiourea, 2 % CHAPS,
0.002 % BPB, 0.5 % ampholytes
IPG, 2-DE, DIGE labeling,
CBB staining MALDI-
QTOF MS/MS,
ESI-QTOF MS/MS.
-
[
21
]
Seed fi lling
stage
50 % phenol, 0.45 M sucrose, 5 mM
EDTA, 0.2 % 2-ME, 50 mM Tris-
HCl pH 8.8
8 M urea, 2 M thiourea, 2 % CHAPS,
2 % Triton X-100, 50 mM DTT,
ampholytes
IPG, 2-DE, MALDI-TOF
MS
679
[
23
]
50 % phenol, 0.45 M sucrose, 5 mM
EDTA, 0.2 % 2-ME, 50 mM Tris-
HCl pH 8.8
8 M urea, 2 M thiourea, 4 % CHAPS,
2 % Triton X-100, 50 mM DTT,
ampholytes
IPG, 2-DE, Sec-MudPIT,
nESI-LC-MS/MS
>900
[
26
]
TCA
trichloroacetic acid,
BPB
bromophenol blue,
CBB
Coomassie brilliant blue,
Sec-MudPIT
semicontinuous multidimensional protein identifi cation technology,
PMSF
phe-
nyl methyl sulfonyl fl uoride,
2-ME
2-mercaptoethanol
