Biology Reference
In-Depth Information
Chapter 23
Soybean Proteomics
Zahed Hossain and Setsuko Komatsu
Abstract
Soybean, the world's most widely grown seed legume, is an important global source of vegetable oil and
protein. Though, complete draft genome sequence of soybean is now available, but functional genomics
studies remain in their infancy, as this agricultural legume species exhibits genetic constrains like genome
duplications and self-incompatibilities. The techniques of proteomics provide much powerful tool for
functional analysis of soybean. In the present review, an attempt has been made to summarize all signifi cant
contributions in the fi eld of soybean proteomics. Special emphasis is given to subcellular proteomics in
response to abiotic stresses for better understanding molecular basis of acquisition of stress tolerance
mechanism. Detailed protocols of protein extraction, solubilization, fractionation of subcellular organelle,
and proteins identifi cation are explained for soybean proteomics. All this information would not only
enrich us in understanding the plants response to environmental stressors but would also enable us to
design genetically engineered stress tolerant soybean.
Key words Soybean, Proteomics, Methodology, Stress
1
Introduction
In the present “omics” era, proteomics has gained ample popularity
over the genome-based technologies as it directly deals with the
functional translated part of the genome. The journey of modern
day proteomics started in 1975 with the establishment of high-
resolution two-dimensional gel electrophoresis (2-DE) technique
by O'Farrell [ 1 ]. Introduction of immobilized pH gradient strips
[ 2 , 3 ] followed by advancement in mass spectrometry (MS) has
made proteomics a fast, sensitive and reliable technique for separa-
tion, identifi cation and characterization of complex protein
mixtures.
Soybean, one of the most important global sources of vegetable
oil and protein, is the fi rst major crop legume species with a pub-
lished complete draft genome sequence [ 4 ]. However, improve-
ments in the functional genomics studies on soybean are still in
their infancy, as this agricultural legume species exhibits genome
duplications and self-incompatibilities with a long generation time.
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