Biology Reference
In-Depth Information
Fig. 1
Application of the Selpex approach to a 120- and 30-min LC-MS analysis. Mean ion intensity of three
biological replicates for (
a
) target
m/z
during 120-min shotgun LC-MS DDP scan analysis, (
b
) 120-min shotgun
LC-MS FS analysis, (
c
) 30-min shotgun LC-MS FS analysis
high-abundance peptides might be dampened. However, the FS
analysis enhances ion count on MS1 level that is reduced during a
DDP MS analysis and therefore enables signifi cant reduction of
MS runtime (Fig.
1
). Runtime for the Selpex approach is linear to
the number of samples. The analysis of three replicates including
ProtMAX processing and identifi cation time took about 5 min (
see
Note 7
). The list of selected peptide targets is very fl exible, is easy
to extend, and can be adapted according to specifi c goals.
